Cereal Chem 59:488 - 492.  |  VIEW ARTICLE
High-Performance Aqueous Gel Permeation Chromatographic Analysis of Beta-Limit Dextrin Hydrolysis by Malted Hard Red Spring Wheat, Malted Durum Wheat, and Fungal (Aspergillus oryzae) Alpha-Amylases.

J. E. Kruger and B. A. Marchylo. Copyright 1982 by the American Association of Cereal Chemists, Inc. 

High-performance aqueous gel permeation chromatography using a Spherogel-TSK 3000 SW column with an exclusion limit of 1.5 x 10(5) daltons was used to analyze the molecular weight distribution of products formed by alpha-amylase catalyzed hydrolysis of beta-limit dextrin. Products formed by alpha-amylases purified from malted hard red spring wheat (Triticum aestivum L. cv. Neepawa), malted durum wheat (Triticum durum cv. Wascana), and fungal (Aspergillus oryzae) sources were studied and compared. The breakdown of beta-limit dextrin proceeded in stages. Initially, the beta-limit dextrin solution exhibited a loss in turbidity concomitant with rapid production of high molecular weight dextrin products larger than 1.5 x 10(5) daltons and little production of low molecular weight dextrins and sugars. This was followed by formation of intermediate molecular weight products that slowly decreased in size concurrent with a progressive buildup of low molecular weight sugars and oligosaccharides. Malted hard red spring and durum wheat alpha-amylase hydrolysis appeared identical but differed from that effected by fungal alpha- amylase.