Cereal Chem 55:146 - 156. | VIEW
Durum Wheat Alpha-Amylases: Isolation and Purification.
J. R. Warchalewski and R. Tkachuk. Copyright 1978 by the American Association of Cereal Chemists, Inc.
Alpha-Amylase was isolated in approximately 7% yields from germinated durum wheat. The optimum isolation conditions were: extraction for 4 hr with 0.001M calcium acetate at 4 C, heat treatment for 10 min. at 70 C, fractionation with 35 to 50% acetone, glycogen complexing in 40% ethanol, and ultrafiltration followed by chromatography on DEAE-cellulose. The chromatography indicated that seven alpha-amylases were present. Polyacrylamide gel electrophoresis showed that germinated durum wheat has eight alpha- amylase components. The eight alpha-amylases that were found by electrophoresis in both germinated durum and germinated hard red spring wheat had similar mobilities.