Cereal Chem 59:178 - 181. | VIEW
An Improved Method for Standardizing Polyacrylamide Gel Electrophoresis of Wheat Gliadin Proteins.
G. L. Lookhart, B. L. Jones, S. B. Hall, and K. F. Finney. Copyright 1982 by the American Association of Cereal Chemists, Inc.
Because different methodologies for polyacrylamide gel electrophoresis (PAGE) analysis precluded direct comparisons of results, we developed a standardized method for PAGE analysis of wheat gliadin extracts. Commercially available equipment and reagents were used. The standardized method should reduce the deleterious effects of the variables (purity and concentration of aluminum lactate, the type of gel former, and the method of gel preparation) and enhance the day-to-day reproducibility of the gliadin separations. The method was adjusted to allow maximum resolution of the gliadin protein bands.