Cereal Chem 53:890 - 901.  |  VIEW ARTICLE
Endosperm Protein Formation During Kernel Development of Wild Type and a High-Lysine Barley Mutant.

A. Brandt. Copyright 1976 by the American Association of Cereal Chemists, Inc. 

The formation of barley endosperm proteins was followed by amino acid analysis and SDS-polyacrylamide gel eletrophoresis during kernel development of wild type barley and the high-lysine mutant, Risphi No. 1508. In the mutant, only minor differences in the amino acid composition and electrophoretic patterns of the albumins and the globulins, respectively, were observed, but three times more free amino acids were present at all stages of endosperm development compared to the wild type. Hordein formation was severely impaired and the synthesis of two components of the fraction was inhibited in the mutant. The high-lysine content of the mutant endosperm is due to this reduction in the lysine-poor hordeins, a reduction of lysine- poor components of the glutelin fraction, and a compensating increase in the amount of lysine-rich glutelins as well as free lysine.