Cereal Chem 49:514 - 521. | VIEW
Determination of Added Lysine in Fortified Wheat and Bulgur.
J. W. Finley, R. E. Ferrel, and D. A. Fellers. Copyright 1972 by the American Association of Cereal Chemists, Inc.
A colorimetric procedure for determination of added lysine-hydrochloride in wheat and bulgur is described. The method is based on quantitative conversion of the alpha-amino group of free lysine to a copper complex, followed by reaction with 1-fluoro-2,4-dinitrobenzene. The solution is acidified with dilute hydrochloric acid and thrice extracted with diethyl ether; the aqueous solution is clarified by addition of ethanol and filtration. Absorbance of the resultant epsilon-dinitrophenyl lysine is measured at 390 nm.