Cereal Chem 48:512 - 522. | VIEW
Purification and Some Properties of Malted-Wheat BAPA-ase.
J. E. Kruger. Copyright 1971 by the American Association of Cereal Chemists, Inc.
A peptide hydrolase from malted wheat, hydrolyzing the synthetic substrate benzoyl-arginine-p-nitroanilide, has been purified by ammonium sulfate fractionation, DEAE-cellulose chromatography, and preparative disc electrophoresis. The isoelectric point, molecular weight, pH optimum, thermal stability, and effects of various chemicals on the activation or inhibition of this enzyme were determined. The enzyme had negligible ability to degrade azocasein, hemoglobin, or gelatin.